Review




Structured Review

Promega pcat-basic plasmid
Pcat Basic Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat+basic+plasmid/pcat+basic+plasmid/pmc02584773-49-1-6
Average 90 stars, based on 1 article reviews
pcat-basic plasmid - by Bioz Stars, 2026-09
90/100 stars

Images

Related Articles

Plasmid Preparation:

Article Title: Glucosamine suppresses proliferation of human prostate carcinoma DU145 cells through inhibition of STAT3 signaling
Article Snippet: After washing, filters were exposed for autoradiography at -70°C with a BioMax screen (Eastman Kodak, Rochester, NY). .. The human CAT reporter plasmid was constructed by inserting a 2.7 kB PCR fragment of the human p21 promoter region (-2583/+110) in the pCAT Basic plasmid (Promega Corporation, Madison, WI). ..

Article Title: Regulation of murine cytochrome oxidase Vb gene expression in different tissues and during myogenesis. Role of a YY-1 factor-binding negative enhancer.
Article Snippet: .. Plasmid Constructs—The 59 deletion promoter constructs were derived from the 2574 CAT DNA (5), which contains the mouse COX Vb sequence 2574 to 140, cloned in pCAT basic plasmid (Promega Biotech Corp.) at theHindIII and blunt ended XbaI sites. .. A series of 59 deletions of the 2574 CAT DNA were generated by the exonuclease III digestion of linearized plasmid, using the “Erase a Base System” (Promega Biotech Corp.).

Article Title: An essential octamer motif in the mb-1 (Igα) promoter
Article Snippet: The mb-1 (Iga) gene is B cell-specific and expressed throughout B cell maturation.. In combination with B29 (Igb) and surface immunoglobulin mb-1 comprises the B cell receptor complex (BCR).. The murine mb-1 promoter has been characterized to depend on the trans-acting transcription factors; Sp1, ets, lkaros, and EBF for full promoter activity.

Article Title: Hepatitis B virus X protein transactivates the inducible nitric oxide synthase promoter.
Article Snippet: The capability of hepatitis B virus (HBV) to increase the transcription of the human hepatic inducible nitric oxide synthase (iNOS) by transactivating its promoter has been studied.. We have observed by reverse-transcription polymerase chain reaction (RT-PCR) that although the mRNA for the iNOS was almost undetectable in the human hepatoblastoma cell line, HepG2, it was constitutively expressed in the 2.2.15 cell line (a derivative of the HepG2 that produces complete HBV particles).. Transfection of HepG2 and 2.2.15 cells with the p1iNOS-CAT plasmid (containing a 1.1-kb fragment of the iNOS promoter) resulted in an increase in chloramphenicol acetyl transferase (CAT) activity in 2.2.15 cells.

Article Title: Polymorphism in the Y box controls level of cytokine-mediated expression of HLA-DRB1 genes.
Article Snippet: The HLA class II molecules play an important role in immune response.. The quality of immune response is dependent not only on the polymorphisms in the class II molecules, but also on the level of their cellsurface expression.. In fact, it has been demonstrated that differences in the level of expression of DRB1 and DRB3 genes restricted and activated distinct CD4π T lymphocytes.

Article Title: Cyclic adenosine 3',5'-monophosphate(cAMP)/cAMP-responsive element modulator (CREM)-dependent regulation of cholesterogenic lanosterol 14alpha-demethylase (CYP51) in spermatids.
Article Snippet: Institute of Biochemistry (D.R., M.F.). Medical Center for Molecular Biology Medical Faculty University of Ljubljana 1000 Ljubljana, Slovenia Institute de Génétique et de Biologie Moléculaire et Cellulaire (G.M.F., P.S.-C.) Centre Nationale de la Recherche Scientifique-INSERM-ULP 67404 Illkrich Strasbourg, France Department of Biochemistry (M.R.W.). Vanderbilt University School of Medicine Nashville, Tennessee 37232-0146

Construct:

Article Title: Glucosamine suppresses proliferation of human prostate carcinoma DU145 cells through inhibition of STAT3 signaling
Article Snippet: After washing, filters were exposed for autoradiography at -70°C with a BioMax screen (Eastman Kodak, Rochester, NY). .. The human CAT reporter plasmid was constructed by inserting a 2.7 kB PCR fragment of the human p21 promoter region (-2583/+110) in the pCAT Basic plasmid (Promega Corporation, Madison, WI). ..

Article Title: Regulation of murine cytochrome oxidase Vb gene expression in different tissues and during myogenesis. Role of a YY-1 factor-binding negative enhancer.
Article Snippet: .. Plasmid Constructs—The 59 deletion promoter constructs were derived from the 2574 CAT DNA (5), which contains the mouse COX Vb sequence 2574 to 140, cloned in pCAT basic plasmid (Promega Biotech Corp.) at theHindIII and blunt ended XbaI sites. .. A series of 59 deletions of the 2574 CAT DNA were generated by the exonuclease III digestion of linearized plasmid, using the “Erase a Base System” (Promega Biotech Corp.).

Polymerase Chain Reaction:

Article Title: Glucosamine suppresses proliferation of human prostate carcinoma DU145 cells through inhibition of STAT3 signaling
Article Snippet: After washing, filters were exposed for autoradiography at -70°C with a BioMax screen (Eastman Kodak, Rochester, NY). .. The human CAT reporter plasmid was constructed by inserting a 2.7 kB PCR fragment of the human p21 promoter region (-2583/+110) in the pCAT Basic plasmid (Promega Corporation, Madison, WI). ..

Derivative Assay:

Article Title: Regulation of murine cytochrome oxidase Vb gene expression in different tissues and during myogenesis. Role of a YY-1 factor-binding negative enhancer.
Article Snippet: .. Plasmid Constructs—The 59 deletion promoter constructs were derived from the 2574 CAT DNA (5), which contains the mouse COX Vb sequence 2574 to 140, cloned in pCAT basic plasmid (Promega Biotech Corp.) at theHindIII and blunt ended XbaI sites. .. A series of 59 deletions of the 2574 CAT DNA were generated by the exonuclease III digestion of linearized plasmid, using the “Erase a Base System” (Promega Biotech Corp.).

Sequencing:

Article Title: Regulation of murine cytochrome oxidase Vb gene expression in different tissues and during myogenesis. Role of a YY-1 factor-binding negative enhancer.
Article Snippet: .. Plasmid Constructs—The 59 deletion promoter constructs were derived from the 2574 CAT DNA (5), which contains the mouse COX Vb sequence 2574 to 140, cloned in pCAT basic plasmid (Promega Biotech Corp.) at theHindIII and blunt ended XbaI sites. .. A series of 59 deletions of the 2574 CAT DNA were generated by the exonuclease III digestion of linearized plasmid, using the “Erase a Base System” (Promega Biotech Corp.).

Clone Assay:

Article Title: Regulation of murine cytochrome oxidase Vb gene expression in different tissues and during myogenesis. Role of a YY-1 factor-binding negative enhancer.
Article Snippet: .. Plasmid Constructs—The 59 deletion promoter constructs were derived from the 2574 CAT DNA (5), which contains the mouse COX Vb sequence 2574 to 140, cloned in pCAT basic plasmid (Promega Biotech Corp.) at theHindIII and blunt ended XbaI sites. .. A series of 59 deletions of the 2574 CAT DNA were generated by the exonuclease III digestion of linearized plasmid, using the “Erase a Base System” (Promega Biotech Corp.).

Article Title: Hepatitis B virus X protein transactivates the inducible nitric oxide synthase promoter.
Article Snippet: The capability of hepatitis B virus (HBV) to increase the transcription of the human hepatic inducible nitric oxide synthase (iNOS) by transactivating its promoter has been studied.. We have observed by reverse-transcription polymerase chain reaction (RT-PCR) that although the mRNA for the iNOS was almost undetectable in the human hepatoblastoma cell line, HepG2, it was constitutively expressed in the 2.2.15 cell line (a derivative of the HepG2 that produces complete HBV particles).. Transfection of HepG2 and 2.2.15 cells with the p1iNOS-CAT plasmid (containing a 1.1-kb fragment of the iNOS promoter) resulted in an increase in chloramphenicol acetyl transferase (CAT) activity in 2.2.15 cells.

Article Title: Cyclic adenosine 3',5'-monophosphate(cAMP)/cAMP-responsive element modulator (CREM)-dependent regulation of cholesterogenic lanosterol 14alpha-demethylase (CYP51) in spermatids.
Article Snippet: Institute of Biochemistry (D.R., M.F.). Medical Center for Molecular Biology Medical Faculty University of Ljubljana 1000 Ljubljana, Slovenia Institute de Génétique et de Biologie Moléculaire et Cellulaire (G.M.F., P.S.-C.) Centre Nationale de la Recherche Scientifique-INSERM-ULP 67404 Illkrich Strasbourg, France Department of Biochemistry (M.R.W.). Vanderbilt University School of Medicine Nashville, Tennessee 37232-0146

Mutagenesis:

Article Title: An essential octamer motif in the mb-1 (Igα) promoter
Article Snippet: The mb-1 (Iga) gene is B cell-specific and expressed throughout B cell maturation.. In combination with B29 (Igb) and surface immunoglobulin mb-1 comprises the B cell receptor complex (BCR).. The murine mb-1 promoter has been characterized to depend on the trans-acting transcription factors; Sp1, ets, lkaros, and EBF for full promoter activity.

Chloramphenicol Acetyltransferase Assay:

Article Title: Hepatitis B virus X protein transactivates the inducible nitric oxide synthase promoter.
Article Snippet: The capability of hepatitis B virus (HBV) to increase the transcription of the human hepatic inducible nitric oxide synthase (iNOS) by transactivating its promoter has been studied.. We have observed by reverse-transcription polymerase chain reaction (RT-PCR) that although the mRNA for the iNOS was almost undetectable in the human hepatoblastoma cell line, HepG2, it was constitutively expressed in the 2.2.15 cell line (a derivative of the HepG2 that produces complete HBV particles).. Transfection of HepG2 and 2.2.15 cells with the p1iNOS-CAT plasmid (containing a 1.1-kb fragment of the iNOS promoter) resulted in an increase in chloramphenicol acetyl transferase (CAT) activity in 2.2.15 cells.

other:

Article Title: A Novel Transcriptional Initiator Activity of the GABP Factor Binding ets Sequence Repeat From the Murine Cytochrome c Oxidase Vb Gene
Article Snippet: All constructions were done in an enhancerless and promoterless CAT basic plasmid (pCAT basic, Promega Biotech Corporation).



Similar Products

90
Promega pcat-basic plasmid
Pcat Basic Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat+basic+plasmid/pcat+basic+plasmid/pmc02584773-49-1-6
Average 90 stars, based on 1 article reviews
pcat-basic plasmid - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Promega pcat basic plasmid
Pcat Basic Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat+basic+plasmid/pcat+basic+plasmid/pmc06138013-85-8-14
Average 90 stars, based on 1 article reviews
pcat basic plasmid - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Promega pcat-basic expression plasmid
Pcat Basic Expression Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat+basic+plasmid/pcat/pmc03286191-351-11-14
Average 90 stars, based on 1 article reviews
pcat-basic expression plasmid - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Promega plasmid pcat basic
Plasmid Pcat Basic, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat+basic+plasmid/pcat+basic+plasmid/pm19912882-67-1-22
Average 90 stars, based on 1 article reviews
plasmid pcat basic - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Promega plasmid pcat 3-basic
Plasmid Pcat 3 Basic, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat+basic+plasmid/pgl3+basic/pm20493845-49-13-16
Average 90 stars, based on 1 article reviews
plasmid pcat 3-basic - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Promega pcat-basic reporter plasmid
Pcat Basic Reporter Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat+basic+plasmid/pcat/pm17406067-69-24-27
Average 90 stars, based on 1 article reviews
pcat-basic reporter plasmid - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Promega reporter plasmid pcat-basic
Reporter Plasmid Pcat Basic, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat+basic+plasmid/pcat/10__1128_slash_jvi__02319___06-65-12-15
Average 90 stars, based on 1 article reviews
reporter plasmid pcat-basic - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results